MICHELANGELO ALOISIO

APPLICATION OF A NEW MOLECULAR APPROACH BASED ON NEXT GENERATION SEQUENCING TO MONITOR THE CHIMERISM POST ALLOGENEIC HSCT

Supervisor:

ROBERTA BULLA

GIOVANNI MARIA SEVERINI

RUSDINA BTE LADJU

The Importance of Death Receptor Pathway in Hepatocellular Carcinoma and It's Potential in the Development of Oligonucleotide Nanomedicine

Supervisor:

CLAUDIO TIRIBELLI

ROSSELLA BALDINI

UNDERSTANDING THE MOLECULAR MECHANISMS UNDERLYING THE PATHOGENESIS OF OPITZ G/BBB SYNDROME EXPLOITING THE Mid1 KNOCK-OUT MOUSE MODEL

Supervisor:

GERMANA MERONI

MARCO DISTEFANO

Functional characterization of the biofilm exopolysaccharide produced by Burkholderia multivorans C1576

Supervisor:

PAOLA CESCUTTI

MARGHERITA DEGASPERI

Optimization of BMAP-18 an anti-infective peptide for the treatment of pulmonary infection

Supervisor:

MARCO SCOCCHI

MASSIMO ROSSO

Oct4 pseudogene lncRNA: dissecting its role in embryonic stem cell differentiation and epigenetic silencing of its ancestral gene in trans

Supervisor:

STEFAN SCHOEFTNER

ROBERTA BENETTI

SERENA RIZZO

IDENTIFICATION AND PRODUCTION OF RECOMBINANT ANTIBODIES TARGETING WEST NILE VIRUS THROUGH NAÏVE AND IMMUNIZED PHAGE-DISPLAY LIBRARIES

Supervisor:

DANIELE SBLATTERO

BARBARA SALIS

STARD3 and the identification of new cholesterol transport inhibitor

Supervisor:

FLAVIO RIZZOLIO

GABRIELE GRASSI

ROMANA VIDERGAR

Complement component C1q and hyaluronic acid act as novel "signaling complex" in malignant pleural mesothelioma microenvironment to promote cancer progression

Supervisor:

ROBERTA BULLA

ANNIE ZAPPONE

Role of SFPQ function in RNA:DNA hybrids related genomic instability in telomeres

Supervisor:

STEFAN SCHOEFTNER

OTTAVIA MARIA ROGGERO

A SYSTEMS PHARMACOLOGY APPROACH FOR INNOVATIVE TREATMENTS TO PROMOTE RECOVERY OF NEURONAL ATROPHY IN RETT SYNDROME

Supervisor:

ENRICO TONGIORGI

LUCA GRISETTI

The Role of Aurora Kinase A in the Development of Hepatocellular Carcinoma and in the Regulation of Programmed Death-Ligand 1

Supervisor:

CLAUDIO TIRIBELLI

DEVIS PASCUT

RICCARDO RUGGERI

Calcium channels and integrins in cortical synaptic transmission: relevance for new therapeutical targets in epilepsy

Supervisor:

LORENZO ANGELO CINGOLANI

LUCREZIA CAMICIA

The role of Multimerin-2 in pericyte recruitment and vascular stability

Supervisor:

MAURIZIO ORLANDINI

DAVIDE VIGETTI

SERENA MAIOCCHI

High throughput screening to model malignant pleural mesothelioma (MPM) pathogenesis: a drug repurposing approach to develop new combination therapies.

Supervisor:

MARCO CONFALONIERI

FEDERICA DATTOLA

Host-directed therapy targeting proteostasis for Tick-borne Encephalitis virus drug discovery

Supervisor:

ALESSANDRO MARCELLO

ALESSANDRO FERRANDO

Recruitment of H3.3 histone chaperon activity to R-loops by the Splicing-Factor Proline and Arginine Rich (SFPQ) stabilizes repetitive elements in cancer cells

Supervisor:

STEFAN SCHOEFTNER

LICIO COLLAVIN

ALESSIA PALDINO

TITIN DOWNREGULATION AFFECTS CARDIOMYOCYTES PROLIFERATION

Supervisor:

GIANFRANCO SINAGRA

ELENA PALMIERI

Investigation of alternative technologies for the development of polysaccharide-based vaccines

Supervisor:

PAOLA CESCUTTI

NICOLA PAVAN

NEW PERSPECTIVE IN PROSTATE CANCER DIAGNOSIS AND TREATMENT: A PIVOTAL STUDY ON EEF1A1 PROTEIN LEVELS AS POTENTIAL THERAPEUTIC TARGET AND LIQUID BIOPSY ON CFDNA OF EEF1A1, CFRNA EEF1A1 AND CFDI OF LINE-1 AS NEW DIAGNOSTIC AND PROGNOSTIC BIOMARKERS

Supervisor:

ALICE TURDO

ALCHIEDE SIMONATO

ALICJA KRAWCZUN-RYGMACZEWSKA

Understanding the pathogenic mechanisms of the ‘Spastic paraplegia, Intellectual disability, Nystagmus and Obesity’ (SINO) syndrome. SINO is a newly described syndrome characterized by Spastic paraplegia, Intellectual Disability, Nystagmus and Obesity, caused by heterozygous mutations in the KIDINS220 gene. Kidins220 (kinase D interacting substrate of 220kDa) is a multi-functional scaffold protein abundantly expressed across the nervous system. Some KIDINS220 pathogenic mutations result in truncated proteins resembling the isoforms naturally expressed during adulthood. This work examines the molecular effect of KIDINS220 mutations on protein expression and how altered KIDINS220 impacts cell homeostasis in HEK293T and primary neurons. Immunocytochemistry revealed that truncating mutations form aggregates and colocalise with p62 and calnexin both in HEK293T cells and neurons. Investigation of mitochondrial membrane potential revealed adverse effects of the mutant proteins on mitochondrial health. Protein extraction of both soluble and insoluble fractions and western blot analysis confirmed that the mutated protein aggregates, unlike the WT, accumulate in the insoluble fraction suggesting impaired liquid-liquid phase separation (LLPS) mechanism which was further assessed by 1,6-hexanediol treatment. In conclusion, we have shown that mutated KIDINS220 aggregates in cells potentially trapping other proteins and disrupting the intracellular interactions. We hypothesise that the mechanisms behind aggregate formation represent a potential druggable target for treatment of KIDINS220-related pathology. In parallel with the experimental work, we have been working together with clinicians and patients’ families to prepare the first clinical overview of SINO syndrome, which should become the reference for newly diagnosed patients.

Supervisor:

FABRIZIA CESCA

FABIO BENFENATI

ANDREA BALDUIT

Evaluation of the Prognostic Power of Complement System in Malignant Pleural Mesothelioma

Supervisor:

ROBERTA BULLA

ROMAN VUERICH

Investigating the cross-talk between cardiomyocytes and endothelial cells to promote cardiac revascularization and regeneration

Supervisor:

SERENA ZACCHIGNA

CHIARA BONCRISTIANI

Reverting therapy resistance of lung cancer cells by forcing RNA:DNA hybrid formation

Supervisor:

STEFAN SCHOEFTNER

LICIO COLLAVIN

CLARA DEZI

Extracellular matrix stiffening promotes a supercompetitive behavior in mutant p53 pre-neoplastic cells

Supervisor:

GIANNINO DEL SAL

FRANCESCO NAPOLETANO

LAURA COLOMINA ALFARO

HELP-based recombinant biopolymers: a versatile platform to realise antimicrobial interfaces for orthopaedic applications

Supervisor:

ANTONELLA BANDIERA

ALESSANDRO TOSSI

VIVIANA MARIA OPINATO

Role of Mesenchymal Stromal Cells in High-Grade Serous Ovarian Cancer

Supervisor:

CLAUDIO SCHNEIDER

ROBERTO VERARDO

DARIA SICARI

Unveiling a role for mutant p53 in regulation of Unfolded Protein Response

Supervisor:

LICIO COLLAVIN

ROSSELLA ZANIN

HMGA1 AND FOXM1 SYNERGISTICALLY REGULATE A COMMON GENE NETWORK MODULATING THE ANGIOGENESIS IN BREAST CANCER

Supervisor:

SILVIA PEGORARO

GUIDALBERTO MANFIOLETTI

MARZIA RAIMONDI

Regulation of autophagy by Ubiquitin Specific Peptidase 1 (USP1)

Supervisor:

CLAUDIO SCHNEIDER

GIULIA MARIA PIPERNO

The actin-nucleator promoting factor WASp regulates endo-lysosomal maturation and Toll-like receptor 9 signaling in Dendritic Cells.

Supervisor:

FEDERICA BENVENUTI

ELENA VALENTINO

A mutant p53 gain of function in the oncogenic response to insulin

Supervisor:

LICIO COLLAVIN

MANUEL CAPUTO

DEPDC1A, a novel SREBP1 cofactor, regulates fatty acid metabolism in breast cancer

Supervisor:

GIANNINO DEL SAL

FRANCESCA CITRON

An integrated approach identifies mediators of local recurrence in head and neck squamous cell carcinoma

Supervisor:

GUSTAVO BALDASSARRE

BARBARA BELLETTI

INÊS FERNANDES LAGES

Identification of Deubiquitinases (DUBs) associated with TRIM18/MID1

Supervisor:

GERMANA MERONI

ADRIANA DI STASI

The designed antimicrobial peptide B7-005: biocompatibility, mode of action and antimicrobial activity against ESKAPE pathogens

Supervisor:

MARCO SCOCCHI

SALVATORE PARISI

STARD3: A new potential therapeutic target in colorectal cancer

Supervisor:

FLAVIO RIZZOLIO

FEDERICA DI CAMILLO

miR-30d suppresses innate immune signalling in BC cells by attenuating the cGAS/STING/IFN-I pathway

Supervisor:

GIANNINO DEL SAL

GIORGIA BIMBI

Local transport and translation of BDNF following synaptic potentiation

Supervisor:

ENRICO TONGIORGI

LUCIA CELORA

COMBINING BIOINFORMATICS WITH WHOLE-CELL PATCH-CLAMP RECORDINGS TO INVESTIGATE THE ROLE OF αVβ3 INTEGRIN IN MOUSE MODELS OF ASD AND EPILEPSY

Supervisor:

FABRIZIA CESCA

LORENZO ANGELO CINGOLANI

MANUEL LISETTO

BIOCHEMICAL AND FUNCTIONAL CHARACTERIZATION OF ZEBRAFISH TRANSGLUTAMINASE 2 PROTEINS

Supervisor:

DANIELE SBLATTERO

ROSSELLA DE FLORIAN FANIA

Identification and characterization of therapeutic molecules affecting expression levels of the tumor suppressor DAB2IP in cancer

Supervisor:

LICIO COLLAVIN

UTSA BHADURI

On the rise of TRIM8 as a multifunctional workhorse during the course of mitosis

Supervisor:

GIUSEPPE MERLA

NOEL SALVOZA

Translational Approach for the Study of Metabolic-Associated Fatty Liver Disease (MAFLD): A Wide Spectrum Disease

Supervisor:

CLAUDIO TIRIBELLI

NATALIA ROSSO

LORAINE KAY CABRAL

Molecular profiling of liver cancer: Genetic and epigenetic variation analysis for developing potential targets for therapy

Supervisor:

CLAUDIO TIRIBELLI

CAECILIA SUKOWATI

LU XIONG

Role of TRIM32, the ubiquitin ligase mutated in Limb Girdle Muscular Dystrophy R8

Supervisor:

GERMANA MERONI

VALENTINA BUEMI

Telomerase regulation by non-coding RNAs

Supervisor:

STEFAN SCHOEFTNER

MARTINA CUSAN

SIGNIFICANCE OF P27 IN GROWTH AND RESPONSE TO THERAPY OF LUMINAL BREAST CANCER

Supervisor:

BARBARA BELLETTI

ILARIA ANNA PIA VOTO

The Pin1 orthologue Dodo regulates heterochromatin formation and activity of transposable elements in Drosophila germline and neural tissue.

Supervisor:

FIAMMA MANTOVANI

GIANNINO DEL SAL

GIANLUIGI DE BENEDETTO

Characterization of polysaccharide-based vaccines against invasive nontyphoidal Salmonella disease (iNTS)

Supervisor:

PAOLA CESCUTTI

FRANCESCA MICOLI

STEFANO GAMBATO

Cathelicidin host defence peptides in cetartiodactyls – identification and characterization of novel members and modification for exploitation as anti-infective agents

Supervisor:

ALESSANDRO TOSSI

ELENA CAMPANER

A new covalent PIN1 inhibitor selectively targets cancer cells by a dual mechanism of action

Supervisor:

GIANNINO DEL SAL

ELEONORA VIANELLO

In vivo evaluation of biomolecular mechanisms of bilirubin toxicity and pre-clinical therapies.

Supervisor:

SILVIA GAZZIN

CLAUDIO TIRIBELLI

DEBORA STELITANO

GTSE1: a novel TEAD4-E2F1 target gene involved in cell protrusions formation in Triple Negative Breast Cancer

Supervisor:

SILVANO PIAZZA

CLAUDIO SCHNEIDER

MICHELA ABRAMI

Biomedical gels: structure and properties

Supervisor:

GABRIELE GRASSI

VERONICA MARIN

Non-Alcoholic and Alcoholic Fatty Liver disease: two sides of the same coin.

Supervisor:

CLAUDIO TIRIBELLI

NATALIA ROSSO

CARLOTTA PENZO

HMGA1 PROTEINS REGULATE GENE EXPRESSION IN TRIPLE-NEGATIVE BREAST CANCER CELLS THROUGH THE MODULATION OF THE EPIGENETIC CODE

Supervisor:

RICCARDO SGARRA

GUIDALBERTO MANFIOLETTI

SRI JAYANTI

THE JANUS FACE OF BILIRUBIN: FROM NEONATAL TOXICITY TO THE POTENTIAL PROTECTION IN NEURODEGENERATIVE DISEASES

Supervisor:

SILVIA GAZZIN

CLAUDIO TIRIBELLI

FILIPPO VIT

The intraclonal diversification of IGHV genes in chronic lymphocytic leukemia: from a bioinformatic approach to the clinics.

Supervisor:

VALTER GATTEI

LORENA MUSCO

p53 context dependent role of Mir-9/KLF5/Sp1 axis in modulating response to therapy in Head and Neck Squamous Cell Carcinoma.

Supervisor:

GUSTAVO BALDASSARRE

ALICE GAMBELLI

ITGA6 is a key molecule in driving metastatization of platinum resistant epithelial ovarian cancer

Supervisor:

GUSTAVO BALDASSARRE

MARIA CONCETTA VOLPE

miR-200c prevents and reverts Lung fibrosis by down regulating Flt1 and promoting lung regeneration

Supervisor:

MARCO CONFALONIERI

JAVAD KARIMBAYLI

Dissecting the role of CDK17 in Epithelial Ovarian Cancer

Supervisor:

GUIDALBERTO MANFIOLETTI

GUSTAVO BALDASSARRE

FERAS KHARRAT

Role of Acylated Ghrelin in Adipose Tissue Metabolism and Investigation of Its Interaction with O-GlcNAc Transferase

Supervisor:

ROCCO BARAZZONI

FRANCESCA DI CRISTINO

The stress-activated kinase p38MAPK contributes to ATF6 activation and resistance to ER stress in cancer cells with mutant p53

Supervisor:

LICIO COLLAVIN

FRANCESCA RUSSO

Implication of p27 kip1 in luminal breast cancer onset and progression

Supervisor:

BARBARA BELLETTI

GUSTAVO BALDASSARRE

CAMILLA TOMBARI

Role of mutant p53 as mechanosensitive regulator of amino-acid metabolism in breast cancer

Supervisor:

GIANNINO DEL SAL

MARIA MICHELINA RASO

Characterization and immunogenicity of O-Antigen based Shigella Generalized Modules for Membrane Antigens vaccines

Supervisor:

FRANCESCA MICOLI

PAOLA CESCUTTI

MARTINA MASCARO

Role of TRIM18, the Opitz Syndrome gene product, in the control of ciliogenesis through autophagy

Supervisor:

GERMANA MERONI

MAGUIE EL BOUSTANI

AP3M2, NEW MOLECULAR TARGET IN COLORECTAL CANCER THERAPY

Supervisor:

GABRIELE GRASSI

RICCARDO SOLA

Towards the design of peptide antibiotics: new insights from synthetic analogues and from natural sources

Supervisor:

MARCO SCOCCHI

PAMELA VENEZIANO BROCCIA

Role of RNA:DNA hybrids management in controlling mitotic chromosome structure in human cancer cells

Supervisor:

STEFAN SCHOEFTNER

ANNALISA BIANCO

The mechanism of bilirubin protection against cellular metabolic damage

Supervisor:

CLAUDIO TIRIBELLI

ALICE COSTA

Dissecting the role of G9a/GLP histone methyltransferases in Platinum-resistant ovarian cancer

Supervisor:

GUSTAVO BALDASSARRE

SARA PETROSINO

HMGA1 MODULATES THE CELL CYCLE- COORDINATED HISTONES EXPRESSION, REDUCING EPIRUBICIN CHEMORESISTANCE IN ER-TNBC CELLS

Supervisor:

GUIDALBERTO MANFIOLETTI

ROBERTA CAPORALE

MODULATION OF ADIPOSE TISSUE METABOLISM BY UNACYLATED GHRELIN AND ITS RELEVANCE IN OBESITY

Supervisor:

ROCCO BARAZZONI

MATTIA APOLLONIO

Regulation and function of tumor suppressor DAB2IP in the cellular response to mechanical inputs

Supervisor:

LICIO COLLAVIN

MUHAMMAD YOGI PRATAMA

TRANSLATIONAL STUDIES ON MICRORNA IN HEPATOCELLULAR CARCINOMA: FROM PREDICTIVE AND PROGNOSTIC BIOMARKERS TO MOLECULAR EFFECTORS

Supervisor:

CLAUDIO TIRIBELLI

EMANUELA RIZZO

Characterization of anti-transglutaminase 2 (TG2) antibodies from Celiac patients by an integrated experimental and computational approach

Supervisor:

DANIELE SBLATTERO

REBECCA BERTOLIO

Sterol Regulatory Element Binding Protein couples mechanical cues to lipid metabolism

Supervisor:

GIANNINO DEL SAL

JAVIER FLORES GUTIÉRREZ

Mirtazapine for Rett syndrome: a candidate to improve quality of life

Supervisor:

ENRICO TONGIORGI

FRANCESCA MARANO

Optimization of a pipeline for the development of recombinant monoclonal antibodies for diagnostics

Supervisor:

DANIELE SBLATTERO

NAVID SOBHANI

The predictive role of the immune system for response to therapy and survival in patients with solid tumors.

Supervisor:

DANIELE GENERALI

GIORGIA MUNGO

ROLE OF p27Kip1 IN MAMMARY GLAND DEVELOPMENT AND TUMORIGENESIS IN Δ16HER2 MODEL

Supervisor:

GUSTAVO BALDASSARRE

BARBARA BELLETTI

GIANDOMENICO ROVIELLO

Tumour Infiltrating Lymphocytes and Immune-Related Genes as Predictors of Outcome in Pancreatic Adenocarcinoma

Supervisor:

DANIELE GENERALI

ANNA IANZA

VALIDATION OF PREDICTIVE AND PROGNOSTIC BIOMARKERS AS A GUIDE FOR A PERSONALIZED APPROACH IN SOLID TUMOURS

Supervisor:

DANIELE GENERALI

FRANCESCA PERRONE

Development of a novel Polymer-based delivery system for siRNAs to hepatocellular carcinoma cells

Supervisor:

GABRIELE GRASSI

MICHELA SGUBIN

HMGA1-p27-stathmin axis promotes migration in triple-negative breast cancer cells

Supervisor:

GUSTAVO BALDASSARRE

GUIDALBERTO MANFIOLETTI

EDOARDO ALFÌ

The role of Emid2 in cancer invasion and metastasis

Supervisor:

SERENA ZACCHIGNA

LUCA TRIBOLI

Understanding the complexity of the tumor microenvironment through an integrated bioinformatics approach

Supervisor:

GIANNINO DEL SAL

DAVIDE RADAELLI

Metabolomic Profiling in Sudden Cardiac Death: Identification of Potential Biomarkers for ischaemic heart disease without myocardial changes and death due to substance abuse.

Supervisor:

GIANFRANCO SINAGRA

ANNA NESPOLO

Targeting the deubiquitinase USP1 to improve PARP inhibitors response in ovarian cancer

Supervisor:

GUSTAVO BALDASSARRE

REBECCA NAPPINI

Facilitating the development of high-valency glycoconjugate vaccines

Supervisor:

FRANCESCA MICOLI

PAOLA CESCUTTI

RENZO ALFINI

ROBERTO AMADIO

Actin polymerization by WASp limits nuclear envelope rupture and inflammation in macrophages

Supervisor:

FEDERICA BENVENUTI

MARIAGIULIA SPAZZAPAN

Bioengineering approaches for the improvement of ovarian autotransplantation

Supervisor:

ROBERTA BULLA

MARIAGIULIA FATTORINI

Development of an innovative platform for the selection and characterization of new generation monoclonal antibodies

Supervisor:

DANIELE SBLATTERO

GABRIELE DI GIUSTINO

Investigating the molecular diversity of luminal breast cancer arising in young women

Supervisor:

BARBARA BELLETTI

GUSTAVO BALDASSARRE

MICHELA ZARO

Utilization of bacteriophage-derived endoglycosidases to combat infections caused by Klebsiella pneumoniae

Supervisor:

PAOLA CESCUTTI

GRETA CAROBOLANTE

Loss of Multimerin-2 associates with YAP/TAZ activation affecting the endothelial cell barrier and impinging on immune cell transmigration

Supervisor:

MAURIZIO MONGIAT

GUSTAVO BALDASSARRE

JESSICA MUIÀ

Investigating Sex-Specific Effects of Cortical β3 Integrin on Autism and Epilepsy: A Behavioral Characterization Study

Supervisor:

LORENZO ANGELO CINGOLANI